Human Ran guanine nucleotide release factor, RANGRF ELISA KIT

Product Name:Human Ran guanine nucleotide release factor, RANGRF ELISA KIT
Packing:96T

Catalog No.:ELI-23200h

Gene Name:RANGRF

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Ran guanine nucleotide release factor(RANGRF

Alternative Name:RANGRF,Ran guanine nucleotide release factor(RANGRF
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Ran guanine nucleotide release factor, RANGRF ELISA KIT allows for the in vitro quantitative determination of Ran guanine nucleotide release factor(RANGRF concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Ran guanine nucleotide release factor, RANGRF ELISA KIT has been pre-coated with an Ran guanine nucleotide release factor(RANGRF antibody specific to Ran guanine nucleotide release factor(RANGRF .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ran guanine nucleotide release factor(RANGRF and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ran guanine nucleotide release factor(RANGRF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ran guanine nucleotide release factor(RANGRF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!