Human TRAF- interacting protein, TRAIP ELISA KIT Product Name:Human TRAF- interacting protein, TRAIP ELISA KIT
Packing:96T
Catalog No.:ELI-23139h
Gene Name:TRAIP
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:TRAF-interacting protein(TRAIP
Alternative Name:TRAIP,TRAF-interacting protein(TRAIP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human TRAF- interacting protein, TRAIP ELISA KIT allows for the in vitro quantitative determination of TRAF-interacting protein(TRAIP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human TRAF- interacting protein, TRAIP ELISA KIT has been pre-coated with an TRAF-interacting protein(TRAIP antibody specific to TRAF-interacting protein(TRAIP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TRAF-interacting protein(TRAIP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TRAF-interacting protein(TRAIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of TRAF-interacting protein(TRAIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!