Human Acetolactate synthase- like protein, ILVBL ELISA KIT

Human Acetolactate synthase- like protein, ILVBL ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

2.2.1.-,Acetolactate synthase-like protein,IlvB-like protein, ILVBL,209L8,AHAS,ILV2H,acetolactate synthase homolog,acetolactate synthase-like protein,ilvB-like protein,ilvB (bacterial acetolactate synthase)-like

Search name

Human 2.2.1.- ELISA KIT ,Human Acetolactate synthase-like protein) ELISA KIT ,Human IlvB-like protein ELISA KIT ,Human  ILVBL ELISA KIT ,Human 209L8 ELISA KIT ,Human AHAS ELISA KIT ,Human ILV2H ELISA KIT ,Human acetolactate synthase homolog ELISA KIT ,Human acetolactate synthase-like protein ELISA KIT ,Human ilvB-like protein ELISA KIT ,Human ilvB (bacterial acetolactate synthase)-like ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Acetolactate synthase-like protein concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetolactate synthase-like protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Acetolactate synthase-like protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Acetolactate synthase-like protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Acetolactate synthase-like protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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