Human Paired mesoderm homeobox protein 2A, PHOX2A ELISA KIT Product Name:Human Paired mesoderm homeobox protein 2A, PHOX2A ELISA KIT
Packing:96T
Catalog No.:ELI-16018h
Gene Name:PHOX2A
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Paired mesoderm homeobox protein 2A(PHOX2A
Alternative Name:PHOX2A,Paired mesoderm homeobox protein 2A(PHOX2A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Paired mesoderm homeobox protein 2A, PHOX2A ELISA KIT allows for the in vitro quantitative determination of Paired mesoderm homeobox protein 2A(PHOX2A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Paired mesoderm homeobox protein 2A, PHOX2A ELISA KIT has been pre-coated with an Paired mesoderm homeobox protein 2A(PHOX2A antibody specific to Paired mesoderm homeobox protein 2A(PHOX2A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Paired mesoderm homeobox protein 2A(PHOX2A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Paired mesoderm homeobox protein 2A(PHOX2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Paired mesoderm homeobox protein 2A(PHOX2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!