Human Phospholipase A2, membrane associated, PLA2G2A ELISA KIT

Product Name:Human Phospholipase A2, membrane associated, PLA2G2A ELISA KIT
Packing:96T

Catalog No.:ELI-14699h

Gene Name:PLA2G2A

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Phospholipase A2(membrane associated(PLA2G2A

Alternative Name:PLA2G2A,Phospholipase A2(membrane associated(PLA2G2A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Phospholipase A2, membrane associated, PLA2G2A ELISA KIT allows for the in vitro quantitative determination of Phospholipase A2(membrane associated(PLA2G2A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Phospholipase A2, membrane associated, PLA2G2A ELISA KIT has been pre-coated with an Phospholipase A2(membrane associated(PLA2G2A antibody specific to Phospholipase A2(membrane associated(PLA2G2A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Phospholipase A2(membrane associated(PLA2G2A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Phospholipase A2(membrane associated(PLA2G2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Phospholipase A2(membrane associated(PLA2G2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!