Human HIV Tat- specific factor 1, HTATSF1 ELISA KIT Product Name:Human HIV Tat- specific factor 1, HTATSF1 ELISA KIT
Packing:96T
Catalog No.:ELI-14310h
Gene Name:HTATSF1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:HIV Tat-specific factor 1(HTATSF1
Alternative Name:HTATSF1,HIV Tat-specific factor 1(HTATSF1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human HIV Tat- specific factor 1, HTATSF1 ELISA KIT allows for the in vitro quantitative determination of HIV Tat-specific factor 1(HTATSF1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human HIV Tat- specific factor 1, HTATSF1 ELISA KIT has been pre-coated with an HIV Tat-specific factor 1(HTATSF1 antibody specific to HIV Tat-specific factor 1(HTATSF1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HIV Tat-specific factor 1(HTATSF1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HIV Tat-specific factor 1(HTATSF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of HIV Tat-specific factor 1(HTATSF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!