Human Endonuclease G mitochondrial, ENDOG ELISA KIT

Human Endonuclease G mitochondrial, ENDOG ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ENDOG,endonuclease G,endo G; endonuclease G, mitochondrial; mitochondrial endonuclease G, Endo G

Search name

Human ENDOG ELISA KIT ,Human endonuclease G ELISA KIT ,Human endo G ELISA KIT ,Human endonuclease G mitochondrial ELISA KIT ,Human mitochondrial endonuclease G ELISA KIT ,Human Endo G ELISA KIT  

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Endonuclease G mitochondrial, ENDOG concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ENDOG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ENDOG and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ENDOG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ENDOG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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