Human NBPF21 (Neuroblastoma breakpoint family member 21) ELISA K

Human NBPF21 (Neuroblastoma breakpoint family member 21) ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Human NBPF21 ELISA KIT ,Human Neuroblastoma breakpoint family member 21 ELISA KIT ,Human NBPF21P; pseudogene ELISA KIT

Intended use

Human NBPF21 ELISA kit allows for the in vitro quantitative determination of Human Neuroblastoma breakpoint family member 21 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Human NBPF21 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NBPF21, During the reaction, NBPF21 in the sample or standard competes with a fixed amount of biotin-labeled NBPF21 for sites on a pre-coated Monoclonal antibody specific to NBPF21. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NBPF21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product    Min.Order 5kits

Delivery time 4-6month

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