Human C5a anaphylatoxin chemotactic receptor, CR1 ELISA KIT Product Name:Human C5a anaphylatoxin chemotactic receptor, CR1 ELISA KIT
Packing:96T
Catalog No.:ELI-11560h
Gene Name:CR1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:C5a anaphylatoxin chemotactic receptor(CR1
Alternative Name:CR1,C5a anaphylatoxin chemotactic receptor(CR1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human C5a anaphylatoxin chemotactic receptor, CR1 ELISA KIT allows for the in vitro quantitative determination of C5a anaphylatoxin chemotactic receptor(CR1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human C5a anaphylatoxin chemotactic receptor, CR1 ELISA KIT has been pre-coated with an C5a anaphylatoxin chemotactic receptor(CR1 antibody specific to C5a anaphylatoxin chemotactic receptor(CR1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for C5a anaphylatoxin chemotactic receptor(CR1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C5a anaphylatoxin chemotactic receptor(CR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of C5a anaphylatoxin chemotactic receptor(CR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!