Human Cleavage stimulation factor subunit 1, CSTF1 ELISA KIT Product Name:Human Cleavage stimulation factor subunit 1, CSTF1 ELISA KIT
Packing:96T
Catalog No.:ELI-10131h
Gene Name:CSTF1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cleavage stimulation factor subunit 1(CSTF1
Alternative Name:CSTF1,Cleavage stimulation factor subunit 1(CSTF1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cleavage stimulation factor subunit 1, CSTF1 ELISA KIT allows for the in vitro quantitative determination of Cleavage stimulation factor subunit 1(CSTF1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cleavage stimulation factor subunit 1, CSTF1 ELISA KIT has been pre-coated with an Cleavage stimulation factor subunit 1(CSTF1 antibody specific to Cleavage stimulation factor subunit 1(CSTF1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cleavage stimulation factor subunit 1(CSTF1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cleavage stimulation factor subunit 1(CSTF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cleavage stimulation factor subunit 1(CSTF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!