Human Embryonal Fyn- associated substrate, EFS ELISA KIT

Product Name:Human Embryonal Fyn- associated substrate, EFS ELISA KIT
Packing:96T

Catalog No.:ELI-09615h

Gene Name:EFS

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Embryonal Fyn-associated substrate(EFS

Alternative Name:EFS,Embryonal Fyn-associated substrate(EFS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Embryonal Fyn- associated substrate, EFS ELISA KIT allows for the in vitro quantitative determination of Embryonal Fyn-associated substrate(EFS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Embryonal Fyn- associated substrate, EFS ELISA KIT has been pre-coated with an Embryonal Fyn-associated substrate(EFS antibody specific to Embryonal Fyn-associated substrate(EFS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Embryonal Fyn-associated substrate(EFS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Embryonal Fyn-associated substrate(EFS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Embryonal Fyn-associated substrate(EFS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!