Human Malonyl- CoA decarboxylase, mitochondrial, MLYCD ELISA KIT

Product Name:Human Malonyl- CoA decarboxylase, mitochondrial, MLYCD ELISA KIT
Packing:96T

Catalog No.:ELI-09007h

Gene Name:MLYCD

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Malonyl-CoA decarboxylase(mitochondrial(MLYCD

Alternative Name:MLYCD,Malonyl-CoA decarboxylase(mitochondrial(MLYCD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Malonyl- CoA decarboxylase, mitochondrial, MLYCD ELISA KIT allows for the in vitro quantitative determination of Malonyl-CoA decarboxylase(mitochondrial(MLYCD concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Malonyl- CoA decarboxylase, mitochondrial, MLYCD ELISA KIT has been pre-coated with an Malonyl-CoA decarboxylase(mitochondrial(MLYCD antibody specific to Malonyl-CoA decarboxylase(mitochondrial(MLYCD .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Malonyl-CoA decarboxylase(mitochondrial(MLYCD and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Malonyl-CoA decarboxylase(mitochondrial(MLYCD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Malonyl-CoA decarboxylase(mitochondrial(MLYCD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!