Human Dermatan- sulfate epimerase- like protein, DSEL ELISA KIT Product Name:Human Dermatan- sulfate epimerase- like protein, DSEL ELISA KIT
Packing:96T
Catalog No.:ELI-08823h
Gene Name:DSEL
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Dermatan-sulfate epimerase-like protein(DSEL
Alternative Name:DSEL,Dermatan-sulfate epimerase-like protein(DSEL
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Dermatan- sulfate epimerase- like protein, DSEL ELISA KIT allows for the in vitro quantitative determination of Dermatan-sulfate epimerase-like protein(DSEL concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Dermatan- sulfate epimerase- like protein, DSEL ELISA KIT has been pre-coated with an Dermatan-sulfate epimerase-like protein(DSEL antibody specific to Dermatan-sulfate epimerase-like protein(DSEL .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Dermatan-sulfate epimerase-like protein(DSEL and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dermatan-sulfate epimerase-like protein(DSEL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Dermatan-sulfate epimerase-like protein(DSEL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!