Human Aspartyl aminopeptidase, DNPEP ELISA KIT

Product Name:Human Aspartyl aminopeptidase, DNPEP ELISA KIT
Packing:96T

Catalog No.:ELI-08037h

Gene Name:DNPEP

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Aspartyl aminopeptidase(DNPEP

Alternative Name:DNPEP,Aspartyl aminopeptidase(DNPEP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Aspartyl aminopeptidase, DNPEP ELISA KIT allows for the in vitro quantitative determination of Aspartyl aminopeptidase(DNPEP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Aspartyl aminopeptidase, DNPEP ELISA KIT has been pre-coated with an Aspartyl aminopeptidase(DNPEP antibody specific to Aspartyl aminopeptidase(DNPEP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Aspartyl aminopeptidase(DNPEP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Aspartyl aminopeptidase(DNPEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Aspartyl aminopeptidase(DNPEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!