Human Adipocyte enhancer- binding protein 1, AEBP1 ELISA KIT

Human Adipocyte enhancer- binding protein 1, Aebp1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Adipocyte enhancer-binding protein 1,AE-binding protein 1,Aortic carboxypeptidase-like protein, Aebp1,ACLP; adipocyte enhancer-binding protein 1; aortic carboxypeptidase-like protein; AE binding protein 1

Search name

Human Adipocyte enhancer-binding protein 1 ELISA KIT ,Human AE-binding protein 1 ELISA KIT ,Human Aortic carboxypeptidase-like protein ELISA KIT ,Human Aebp1 ELISA KIT ,Human ACLP ELISA KIT ,Human adipocyte enhancer-binding protein 1 ELISA KIT ,Human aortic carboxypeptidase-like protein ELISA KIT ,Human AE binding protein 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human adipocyte enhancer-binding protein 1,AE-binding protein 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to AE-binding protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AE-binding protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AE-binding protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AE-binding protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.