Human Fumarate hydratase, mitochondrial, FH ELISA KIT

Product Name:Human Fumarate hydratase, mitochondrial, FH ELISA KIT
Packing:96T

Catalog No.:ELI-06207h

Gene Name:FH

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Fumarate hydratase(mitochondrial(FH

Alternative Name:FH,Fumarate hydratase(mitochondrial(FH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Fumarate hydratase, mitochondrial, FH ELISA KIT allows for the in vitro quantitative determination of Fumarate hydratase(mitochondrial(FH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Fumarate hydratase, mitochondrial, FH ELISA KIT has been pre-coated with an Fumarate hydratase(mitochondrial(FH antibody specific to Fumarate hydratase(mitochondrial(FH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Fumarate hydratase(mitochondrial(FH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Fumarate hydratase(mitochondrial(FH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Fumarate hydratase(mitochondrial(FH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!