Human Insulin- degrading enzyme, IDE ELISA KIT

Product Name:Human Insulin- degrading enzyme, IDE ELISA KIT
Packing:96T

Catalog No.:ELI-06091h

Gene Name:IDE

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Insulin-degrading enzyme(IDE

Alternative Name:IDE,Insulin-degrading enzyme(IDE
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Insulin- degrading enzyme, IDE ELISA KIT allows for the in vitro quantitative determination of Insulin-degrading enzyme(IDE concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Insulin- degrading enzyme, IDE ELISA KIT has been pre-coated with an Insulin-degrading enzyme(IDE antibody specific to Insulin-degrading enzyme(IDE .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Insulin-degrading enzyme(IDE and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Insulin-degrading enzyme(IDE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Insulin-degrading enzyme(IDE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!