Human Selenoprotein P, SEPP1 ELISA KIT

Human Selenoprotein P, SEPP1 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Selenoprotein P,SeP, Sepp1,selenoprotein P,SELP; SeP; selenoprotein P plasma 1

 

Search name

Human Sepp1 ELISA KIT ,Human Selenoprotein P ELISA KIT ,Human SeP ELISA KIT ,Human selenoprotein P ELISA KIT ,Human SELP ELISA KIT ,Human SeP ELISA KIT ,Human selenoprotein P plasma 1 ELISA KIT

 

Intended use

Human Sepp1 ELISA KIT allows for the in vitro quantitative determination of human selenoprotein p,SeP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Human Sepp1 ELISA KIT has been pre-coated with an antibody specific to SeP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SeP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SeP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SeP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human Sepp1 ELISA KIT  Range 0.15-10ng/mL

Human Sepp1 ELISA KIT PDF manual download

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