Human Tryptophanyl- tRNA synthetase, cytoplasmic, WARS ELISA KIT Product Name:Human Tryptophanyl- tRNA synthetase, cytoplasmic, WARS ELISA KIT
Packing:96T
Catalog No.:ELI-05670h
Gene Name:WARS
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Tryptophanyl-tRNA synthetase(cytoplasmic(WARS
Alternative Name:WARS,Tryptophanyl-tRNA synthetase(cytoplasmic(WARS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Tryptophanyl- tRNA synthetase, cytoplasmic, WARS ELISA KIT allows for the in vitro quantitative determination of Tryptophanyl-tRNA synthetase(cytoplasmic(WARS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Tryptophanyl- tRNA synthetase, cytoplasmic, WARS ELISA KIT has been pre-coated with an Tryptophanyl-tRNA synthetase(cytoplasmic(WARS antibody specific to Tryptophanyl-tRNA synthetase(cytoplasmic(WARS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Tryptophanyl-tRNA synthetase(cytoplasmic(WARS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tryptophanyl-tRNA synthetase(cytoplasmic(WARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Tryptophanyl-tRNA synthetase(cytoplasmic(WARS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!