Human Neutrophil defensin 1, DEFA1 ELISA KIT

Product Name:Human Neutrophil defensin 1, DEFA1 ELISA KIT
Packing:96T

Catalog No.:ELI-05403h

Gene Name:DEFA1

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Neutrophil defensin 1(DEFA1

Alternative Name:DEFA1,Neutrophil defensin 1(DEFA1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Neutrophil defensin 1, DEFA1 ELISA KIT allows for the in vitro quantitative determination of Neutrophil defensin 1(DEFA1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Neutrophil defensin 1, DEFA1 ELISA KIT has been pre-coated with an Neutrophil defensin 1(DEFA1 antibody specific to Neutrophil defensin 1(DEFA1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Neutrophil defensin 1(DEFA1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neutrophil defensin 1(DEFA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Neutrophil defensin 1(DEFA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!