Human Regulatory- associated protein of mTOR, RPTOR ELISA KIT

Product Name:Human Regulatory- associated protein of mTOR, RPTOR ELISA KIT
Packing:96T

Catalog No.:ELI-05111h

Gene Name:RPTOR

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Regulatory-associated protein of mTOR(RPTOR

Alternative Name:RPTOR,Regulatory-associated protein of mTOR(RPTOR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Regulatory- associated protein of mTOR, RPTOR ELISA KIT allows for the in vitro quantitative determination of Regulatory-associated protein of mTOR(RPTOR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Regulatory- associated protein of mTOR, RPTOR ELISA KIT has been pre-coated with an Regulatory-associated protein of mTOR(RPTOR antibody specific to Regulatory-associated protein of mTOR(RPTOR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Regulatory-associated protein of mTOR(RPTOR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Regulatory-associated protein of mTOR(RPTOR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Regulatory-associated protein of mTOR(RPTOR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!