Human Tyrosine- protein kinase SYK, SYK ELISA KIT Product Name:Human Tyrosine- protein kinase SYK, SYK ELISA KIT
Packing:96T
Catalog No.:ELI-05013h
Gene Name:SYK
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Tyrosine-protein kinase SYK(SYK
Alternative Name:SYK,Tyrosine-protein kinase SYK(SYK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Tyrosine- protein kinase SYK, SYK ELISA KIT allows for the in vitro quantitative determination of Tyrosine-protein kinase SYK(SYK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Tyrosine- protein kinase SYK, SYK ELISA KIT has been pre-coated with an Tyrosine-protein kinase SYK(SYK antibody specific to Tyrosine-protein kinase SYK(SYK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Tyrosine-protein kinase SYK(SYK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tyrosine-protein kinase SYK(SYK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Tyrosine-protein kinase SYK(SYK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!