Human Pancreatic prohormone, PPY ELISA KIT

Human Pancreatic prohormone, PPY ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Pancreatic prohormone,Pancreatic polypeptide,PP, PNP; pancreatic polypeptide Y; prepro-PP,prepropancreatic polypeptide,Pancreatic prohormone,

Search name

Human PPY ELISA KIT, Human Pancreatic prohormone ELISA KIT ,Human Pancreatic polypeptide ELISA KIT ,Human PP ELISA KIT ,Human PNP ELISA KIT ,Human pancreatic polypeptide Y ELISA KIT ,Human prepro-PP ELISA KIT ,Human prepropancreatic polypeptide ELISA KIT ,Human Pancreatic prohormone ELISA KIT

Intended use

Human PPY ELISA KIT allows for the in vitro quantitative determination of human pancreatic prohormone,Pancreatic prohormone concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human PPY ELISA KIT has been pre-coated with an antibody specific to Pancreatic prohormone. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pancreatic prohormone and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pancreatic prohormone, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pancreatic prohormone in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human PPY ELISA KIT Range 49.6-3000pg/ml

Human PPY ELISA KIT PDF manual download

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