Human ALK tyrosine kinase receptor, ALK ELISA KIT Product Name:Human ALK tyrosine kinase receptor, ALK ELISA KIT
Packing:96T
Catalog No.:ELI-03653h
Gene Name:ALK
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:ALK tyrosine kinase receptor(ALK
Alternative Name:ALK,ALK tyrosine kinase receptor(ALK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ALK tyrosine kinase receptor, ALK ELISA KIT allows for the in vitro quantitative determination of ALK tyrosine kinase receptor(ALK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ALK tyrosine kinase receptor, ALK ELISA KIT has been pre-coated with an ALK tyrosine kinase receptor(ALK antibody specific to ALK tyrosine kinase receptor(ALK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ALK tyrosine kinase receptor(ALK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ALK tyrosine kinase receptor(ALK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of ALK tyrosine kinase receptor(ALK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!