Human Lysophosphatidic acid receptor 3, LPAR3 ELISA KIT

Human Lysophosphatidic acid receptor 3, LPAR3 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

LPAR3,EDG7; Edg-7; GPCR; HOFNH30; LP-A3; LPA3; RP4-678I3; LPA receptor 3; LPA receptor EDG7; LPA-3; calcium-mobilizing lysophosphatidic acid receptor LP-A3; endothelial cell differentiation gene 7; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7; lysophosphatidic acid receptor Edg-7; lysophosphatidic acid receptor 3,Lysophosphatidic acid receptor 3,LPA receptor 3,LPA-3,Lysophosphatidic acid receptor Edg-7,

Search name

Human LPAR3 ELISA KIT ,Human  LPA-3 ELISA KIT ,Human EDG7 ELISA KIT ,Human Edg-7 ELISA KIT ,Human GPCR ELISA KIT ,Human HOFNH30 ELISA KIT ,Human LP-A3 ELISA KIT ,Human LPA3 ELISA KIT ,Human RP4-678I3 ELISA KIT ,Human LPA receptor 3 ELISA KIT ,Human LPA receptor EDG7 ELISA KIT ,Human LPA-3 ELISA KIT ,Human calcium-mobilizing lysophosphatidic acid receptor LP-A3 ELISA KIT ,Human endothelial cell differentiation gene 7 ELISA KIT ,Human endothelial differentiation ELISA KIT ,Human  lysophosphatidic acid G-protein-coupled receptor ELISA KIT ,Human  7 ELISA KIT ,Human lysophosphatidic acid receptor Edg-7 ELISA KIT ,Human lysophosphatidic acid receptor 3 ELISA KIT ,Human LPA receptor 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human lysophosphatidic acid receptor 3,LPA receptor 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to LPA receptor 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LPA receptor 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LPA receptor 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LPA receptor 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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