Human Adiponectin, ADIPOQ ELISA KIT

Human adiponectin, ADIPOQ ELISA KIT
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Adiponectin,Gelatin-binding protein,Adipose most abundant gene transcript 1 protein(apM-1),Adipocyte, C1q and collagen
domain-containing protein,30 kDa adipocyte complement-related protein,Adipocyte complement-related 30 kDa protein(ACRP30); ACDC;
ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28; adipose specific collagen-like factor; gelatin-binding protein 28

Search name
Human adiponectin ELISA Kit, Human ADP ELISA Kit, Human Adiponectin,Gelatin-binding protein ELISA Kit, Human Adipose
most abundant gene transcript 1 protein ELISA Kit, Human apM-1 ELISA Kit, Human Adipocyte ELISA Kit, Human C1q and
collagen domain-containing protein ELISA Kit, Human 30 kDa adipocyte complement-related protein ELISA Kit, Human
Adipocyte complement-related 30 kDa protein ELISA Kit, Human ACRP30 ELISA Kit, Human ACDC ELISA Kit, Human ADIPQTL1
ELISA Kit, Human ADPN ELISA Kit, Human APM1 ELISA Kit, Human GBP28 ELISA Kit, Human adipose specific collagen-like
factor ELISA Kit, Human gelatin-binding protein 28 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of human adiponectin,Adiponectin concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Adiponectin and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Adiponectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Adiponectin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

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