Human Granzyme B, GZMB ELISA KIT

Product Name:Human Granzyme B, GZMB ELISA KIT
Packing:96T

Catalog No.:ELI-02160h

Gene Name:GZMB

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Granzyme B(GZMB

Alternative Name:GZMB,Granzyme B(GZMB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Granzyme B, GZMB ELISA KIT allows for the in vitro quantitative determination of Granzyme B(GZMB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Granzyme B, GZMB ELISA KIT has been pre-coated with an Granzyme B(GZMB antibody specific to Granzyme B(GZMB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Granzyme B(GZMB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Granzyme B(GZMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Granzyme B(GZMB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!