Human Desmopressin/ 1- desamino- 8- D- arginine vasopressin ELISA KIT
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Human Ddavp ELISA KIT ,Human Minirin ELISA KIT ,Human Minirin Melt ELISA KIT ,Human Octim ELISA KIT ,Human Stimate ELISA KIT ,Human Desmopressin ELISA KIT ,Human 1-desamino-8-D-arginine vasopressin ELISA KIT
Intended use
Human dDAVP ELISA kit allows for the in vitro quantitative determination of Human Desmopressin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Human dDAVP ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to dDAVP, During the reaction, dDAVP in the sample or standard competes with a fixed amount of biotin-labeled dDAVP for sites on a pre-coated Monoclonal antibody specific to dDAVP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of dDAVP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human dDAVP ELISA kit Range 6.25-400pg/ml
Human dDAVP ELISA kit manual download
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