Human Plasma fibronectin ELISA Kit

Product Name:Human Plasma fibronectin ELISA Kit
Packing:96T

Catalog No.:ELA-E1807h

Gene Name:pFN

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Plasma fibronectin

Alternative Name:pFN,Plasma fibronectin
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Plasma fibronectin ELISA Kit allows for the in vitro quantitative determination of Plasma fibronectin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Plasma fibronectin ELISA Kit has been pre-coated with an Plasma fibronectin antibody specific to Plasma fibronectin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Plasma fibronectin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Plasma fibronectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Plasma fibronectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!