Human guanylate nucleotide binding protein 4 ELISA Kit

Product Name:Human guanylate nucleotide binding protein 4 ELISA Kit
Packing:96T

Catalog No.:ELA-E1772h

Gene Name:Gbp4

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:guanylate nucleotide binding protein 4

Alternative Name:Gbp4,guanylate nucleotide binding protein 4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human guanylate nucleotide binding protein 4 ELISA Kit allows for the in vitro quantitative determination of guanylate nucleotide binding protein 4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human guanylate nucleotide binding protein 4 ELISA Kit has been pre-coated with an guanylate nucleotide binding protein 4 antibody specific to guanylate nucleotide binding protein 4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for guanylate nucleotide binding protein 4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain guanylate nucleotide binding protein 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of guanylate nucleotide binding protein 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!