Human soluble cluster of differentiation 44 variant 9 ELISA Kit Product Name:Human soluble cluster of differentiation 44 variant 9 ELISA Kit
Packing:96T
Catalog No.:ELA-E1654h
Gene Name:sCD44-v9
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:soluble cluster of differentiation 44 variant 9
Alternative Name:sCD44-v9,soluble cluster of differentiation 44 variant 9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human soluble cluster of differentiation 44 variant 9 ELISA Kit allows for the in vitro quantitative determination of soluble cluster of differentiation 44 variant 9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human soluble cluster of differentiation 44 variant 9 ELISA Kit has been pre-coated with an soluble cluster of differentiation 44 variant 9 antibody specific to soluble cluster of differentiation 44 variant 9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for soluble cluster of differentiation 44 variant 9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain soluble cluster of differentiation 44 variant 9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of soluble cluster of differentiation 44 variant 9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!