Human Guanine nucleotide dissociation Inhibitor ELISA Kit

Product Name:Human Guanine nucleotide dissociation Inhibitor ELISA Kit
Packing:96T

Catalog No.:ELA-E1617h

Gene Name:GDI

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Guanine nucleotide dissociation Inhibitor

Alternative Name:GDI,Guanine nucleotide dissociation Inhibitor
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Guanine nucleotide dissociation Inhibitor ELISA Kit allows for the in vitro quantitative determination of Guanine nucleotide dissociation Inhibitor concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Guanine nucleotide dissociation Inhibitor ELISA Kit has been pre-coated with an Guanine nucleotide dissociation Inhibitor antibody specific to Guanine nucleotide dissociation Inhibitor .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Guanine nucleotide dissociation Inhibitor and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Guanine nucleotide dissociation Inhibitor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Guanine nucleotide dissociation Inhibitor in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!