Human Cathelicidin Antimicrobial Peptide ELISA Kit

Human Cathelicidin Antimicrobial Peptide, CAMP ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Cathelicidin antimicrobial peptide,;18 kDa cationic antimicrobial protein(CAP-18,hCAP-18),;
LL-37,CAP-18; CAP18; CRAMP; FALL-39; FALL39; LL37; 18 kDa cationic antimicrobial protein; cathelicidin antimicrobial peptide

Search name
Human Cathelicidin Antimicrobial Peptide ELISA Kit, Human CAMP ELISA Kit, Human 18 kDa cationic antimicrobial protein
ELISA Kit, Human CAP-18 ELISA Kit, Human hCAP-18 ELISA Kit, Human LL-37 ELISA Kit, Human CRAMP ELISA Kit, Human
FALL-39 ELISA Kit, Human FALL39 ELISA Kit, Human LL37 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of human cathelicidin antimicrobial peptide,Cathelicidin
antimicrobial peptide concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Cathelicidin antimicrobial peptide, During the reaction, Cathelicidin antimicrobial peptide in the
sample or standard competes with a fixed amount of biotin-labeled Cathelicidin antimicrobial peptide for sites on a pre-coated Monoclonal
antibody specific to Cathelicidin antimicrobial peptide. Excess conjugate and unbound sample or standard are washed from the plate.
Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution
is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cathelicidin antimicrobial peptide in the
samples is then determined by comparing the O.D. of the samples to the standard curve.