Human SFRP5 ELISA Kit Product Name:Human SFRP5 ELISA Kit
Packing:96T
Catalog No.:ELA-E1434h
Gene Name:SFRP5
Detect Range:1.56-100 ng/mL
Sensitivity:0.98ng/mL
Target Protein Name:Human Secreted frizzled-related protein 5
Alternative Name:Secreted frizzled-related protein 5, sFRP-5, Frizzled-related protein 1b, FRP-1b, Secreted apoptosis-related protein 3, SARP-3, SFRP5, FRP1B, SARP3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human SFRP5 ELISA Kit allows for the in vitro quantitative determination of Human Secreted frizzled-related protein 5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human SFRP5 ELISA Kit has been pre-coated with an SFRP5 antibody specific to Human Secreted frizzled-related protein 5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Secreted frizzled-related protein 5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Secreted frizzled-related protein 5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm