Human NOS1 ELISA Kit

Product Name:Human NOS1 ELISA Kit
Packing:96T

Catalog No.:ELA-E0628h

Gene Name:NOS1

Detect Range:0.156-10 ng/mL

Sensitivity:0.089ng/mL

Target Protein Name:Human Nitric oxide synthase, brain

Alternative Name:Nitric oxide synthase, brain, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, N-NOS, nNOS, Peptidyl-cysteine S-nitrosylase NOS1, bNOS, NOS1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human NOS1 ELISA Kit allows for the in vitro quantitative determination of Human Nitric oxide synthase, brain concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human NOS1 ELISA Kit has been pre-coated with an NOS1 antibody specific to Human Nitric oxide synthase, brain .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Nitric oxide synthase, brain and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Nitric oxide synthase, brain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm