Human oxidized lowdensity lipoprotein antibody ELISA Kit Product Name:Human oxidized lowdensity lipoprotein antibody ELISA Kit
Packing:96T
Catalog No.:ELA-E0617h
Gene Name:OLAb
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:oxidized lowdensity lipoprotein antibody
Alternative Name:OLAb,oxidized lowdensity lipoprotein antibody
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human oxidized lowdensity lipoprotein antibody ELISA Kit allows for the in vitro quantitative determination of oxidized lowdensity lipoprotein antibody concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human oxidized lowdensity lipoprotein antibody ELISA Kit has been pre-coated with an oxidized lowdensity lipoprotein antibody antibody specific to oxidized lowdensity lipoprotein antibody .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for oxidized lowdensity lipoprotein antibody and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain oxidized lowdensity lipoprotein antibody, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of oxidized lowdensity lipoprotein antibody in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!