Human Anti- respiratory syncytial virus IgM ELISA kit Product Name:Human Anti- respiratory syncytial virus IgM ELISA kit
Packing:96T
Catalog No.:ELA-E0367h
Gene Name:RSV IgM
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Anti-respiratory syncytial virus IgM
Alternative Name:RSV IgM,Anti-respiratory syncytial virus IgM
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Anti- respiratory syncytial virus IgM ELISA kit allows for the in vitro quantitative determination of Anti-respiratory syncytial virus IgM concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Anti- respiratory syncytial virus IgM ELISA kit has been pre-coated with an Anti-respiratory syncytial virus IgM antibody specific to Anti-respiratory syncytial virus IgM .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Anti-respiratory syncytial virus IgM and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Anti-respiratory syncytial virus IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Anti-respiratory syncytial virus IgM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!