Human PROK1 ELISA Kit

Product Name:Human PROK1 ELISA Kit
Packing:96T

Catalog No.:ELA-E0024h

Gene Name:PROK1

Detect Range:7.8-500 pg/mL

Sensitivity:2.1 pg/mL

Target Protein Name:Human Prokineticin-1

Alternative Name:Prokineticin-1, Endocrine-gland-derived vascular endothelial growth factor, EG-VEGF, Mambakine, PROK1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PROK1 ELISA Kit allows for the in vitro quantitative determination of Human Prokineticin-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PROK1 ELISA Kit has been pre-coated with an PROK1 antibody specific to Human Prokineticin-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Prokineticin-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Prokineticin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm