Vimentin| Vimentin antibody

货号  PAab09410

蛋白别名  FLJ36605 antibody, VIM antibody, Vimentin

蛋白介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

产品描述

建议稀释比例

WB :1:1000-1:10000;IHC:1:20-1:2000;IF:1:20-1:200

IHC

Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using PAab09410( Vimentin Antibody) at dilution of 1:500.heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Western blot

Jurkat cells were subjected to SDS PAGE followed by western blot with PAab09410( Vimentin Antibody) at dilution of 1:2000(本抗体仅供体外科研用途，不可用于临床诊断!)

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