Rat 3 beta- hydroxysteroid dehydrogenase/Delta 5- - >4- isomeras

Rat 3 beta- hydroxysteroid dehydrogenase/Delta 5- - >4- isomerase, HSD3B ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

HSD3B,HSD3B1,3BETAHSD; HSDB3; HSDB3A; SDR11E1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; delta-5-3-ketosteroid isomerase; hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1; progesterone reductase; short chain dehydrogenase/reductase family 11E, member 1; steroid Delta-isomerase; trophoblast antigen FDO161G; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat 3 beta- hydroxysteroid dehydrogenase/Delta 5- - >4- isomerase, HSD3B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSD3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HSD3B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HSD3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HSD3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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