Rat Deleted in malignant brain tumors 1 protein, DMBT1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DMBT1,GP340; muclin; SAG; deleted in malignant brain tumors 1 protein; glycoprotein 340; gp-340; hensin; salivary agglutinin; surfactant pulmonary-associated D-binding protein; deleted in malignant brain tumors 1
Search name
Rat DMBT1 ELISA KIT ,Rat GP340 ELISA KIT ,Rat muclin ELISA KIT ,Rat SAG ELISA KIT ,Rat deleted in malignant brain tumors 1 protein ELISA KIT ,Rat glycoprotein 340 ELISA KIT ,Rat gp-340 ELISA KIT ,Rat hensin ELISA KIT ,Rat salivary agglutinin ELISA KIT ,Rat surfactant pulmonary-associated D-binding protein ELISA KIT ,Rat deleted in malignant brain tumors 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Deleted in malignant brain tumors 1 protein, DMBT1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DMBT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DMBT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DMBT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DMBT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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