Rat Nucleoside diphosphate kinase B, Nme2 ELISA试剂盒

Product Name:Rat Nucleoside diphosphate kinase B, Nme2 ELISA KIT
Packing:96T

Catalog No.:ELI-20080r

Gene Name:Nme2

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Rat Nucleoside diphosphate kinase B

Alternative Name:Nme2,Rat Nucleoside diphosphate kinase B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Rat Nucleoside diphosphate kinase B, Nme2 ELISA KIT allows for the in vitro quantitative determination ofRat Nucleoside diphosphate kinase B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Rat Nucleoside diphosphate kinase B, Nme2 ELISA KIT has been pre-coated with an Nme2 antibody specific to Rat Nucleoside diphosphate kinase B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rat Nucleoside diphosphate kinase B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Nucleoside diphosphate kinase B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rat Nucleoside diphosphate kinase B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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