Rat Specifically androgen- regulated gene protein, SARG ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SARG, C1orf116,specifically androgen-regulated gene protein; specifically androgen-regulated protein; chromosome 1 open reading frame 116
Search name
Rat SARG ELISA KIT ,Rat C1orf116 ELISA KIT ,Rat specifically androgen-regulated gene protein ELISA KIT ,Rat specifically androgen-regulated protein ELISA KIT ,Rat chromosome 1 open reading frame 116 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Specifically androgen- regulated gene protein, SARG concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SARG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SARG and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SARG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SARG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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