Rat Extracellular superoxide dismutase [Cu-Zn], EC-SOD ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class 1.15.1.1, Superoxide dismutase B, SOD3,EC-SOD, extracellular superoxide dismutase [Cu-Zn],superoxide dismutase 3 extracellular
Search name
Rat Class 1.15.1.1 ELISA KIT ,Rat Superoxide dismutase B ELISA KIT ,Rat SOD3 ELISA KIT ,Rat EC-SOD ELISA KIT ,Rat extracellular superoxide dismutase [Cu-Zn] ELISA KIT ,Rat superoxide dismutase 3 extracellular ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat extracellular superoxide dismutase [cu-zn],EC-SOD concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to EC-SOD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EC-SOD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EC-SOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EC-SOD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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