Rat SH3GL2 (Endophilin- A1) ELISA试剂盒 (CUSTOM)

Rat SH3GL2 (Endophilin- A1) ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Rat Sh3gl2 ELISA KIT ,Rat CNSA2 ELISA KIT ,Rat EEN-B1 ELISA KIT ,Rat SH3D2A ELISA KIT ,Rat SH3P4 ELISA KIT ,Rat Endophilin A1 BAR domain ELISA KIT ,Rat SH3 domain protein 2A ELISA KIT ,Rat SH3 domain-containing GRB2-like protein 2 ELISA KIT ,Rat SH3-domain GRB2-like 2 ELISA KIT ,Rat bA335L15.1 (SH3-domain GRB2-like 2) ELISA KIT ,Rat endophilin-1 ELISA KIT ,Rat SH3 domain containing GRB2 like 2 ELISA KIT

Intended use

Rat SH3GL2 ELISA kit allows for the in vitro quantitative determination of Rat Endophilin- A1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Rat SH3GL2 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SH3GL2, During the reaction, SH3GL2 in the sample or standard competes with a fixed amount of biotin-labeled SH3GL2 for sites on a pre-coated Monoclonal antibody specific to SH3GL2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SH3GL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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