Rat Visceral adipose-specific serine protease inhibitor, vaspin

Rat Visceral adipose-specific serine protease inhibitor, vaspin ELISA Kit

96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms
Serpin A12,Visceral adipose-specific serpin,OL-64,Visceral adipose tissue-derived serine protease inhibitor(Vaspin),;
SERPINA12,visceral adipose-specific SERPIN; visceral adipose tissue-derived serine protease inhibitor; OL-64; serine (or cysteine)
proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; serpin A12; vaspin; serpin peptidase inhibitor, clade A
(alpha-1 antiproteinase, antitrypsin), member 12

Search name
Rat Serpin A12 ELISA Kit, Rat SERPINA12 ELISA Kit, Serpin A12 ELISA Kit, SERPINA12 ELISA Kit, Rat Visceral
adipose-specific serine protease inhibitor ELISA Kit, Rat vaspin ELISA Kit, Visceral adipose-specific serine protease
inhibitor ELISA Kit, vaspin ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat serpin a12,Serpin A12 concentrations in serum, Plasma,
Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serpin A12. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Serpin A12 and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Serpin A12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serpin A12 in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

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