Bovine Mitotic checkpoint protein BUB3, BUB3 ELISA KIT Product Name:Bovine Mitotic checkpoint protein BUB3, BUB3 ELISA KIT
Packing:96T
Catalog No.:ELI-50146b
Gene Name:Bovine Mitotic checkpoint protein BUB3, BUB3
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Mitotic checkpoint protein BUB3, BUB3
Alternative Name:BUB3,Bovine Mitotic checkpoint protein BUB3, BUB3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Mitotic checkpoint protein BUB3, BUB3 ELISA KIT allows for the in vitro quantitative determination of Bovine Mitotic checkpoint protein BUB3, BUB3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Mitotic checkpoint protein BUB3, BUB3 ELISA KIT has been pre-coated with an Bovine Mitotic checkpoint protein BUB3, BUB3 antibody specific to Bovine Mitotic checkpoint protein BUB3, BUB3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Mitotic checkpoint protein BUB3, BUB3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Mitotic checkpoint protein BUB3, BUB3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Mitotic checkpoint protein BUB3, BUB3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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