Bovine Acylamino- acid- releasing enzyme, APEH ELISA KIT

Bovine Acylamino- acid- releasing enzyme, APEH ELISA KIT 
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Acylamino-acid-releasing enzyme(AARE),Oxidized protein hydrolase(OPH),Acyl-peptide hydrolase(APH),Acylaminoacyl-peptidase,
APEH,AARE; ACPH; APH; D3F15S2; D3S48E; DNF15S2; OPH; N-acylaminoacyl-peptide hydrolase; acyl-peptide hydrolase;
acylamino-acid-releasing enzyme; acylaminoacyl-peptidase; oxidized protein hydrolase; acylaminoacyl-peptide hydrolase

Search name
Bovine APEH ELISA KIT,Bovine Acylamino acid releasing enzyme ELISA Kit, Bovine AARE ELISA Kit, Bovine Acylamino-acid-releasing enzym ELISA Kit,
Bovine Oxidized protein hydrolase ELISA Kit, Bovine OPH ELISA Kit, Bovine Acyl-peptide hydrolase ELISA Kit, Bovine APH
ELISA Kit, Bovine Acylaminoacyl-peptidase ELISA Kit, Bovine APEH ELISA Kit, Bovine ACPH ELISA Kit, Bovine D3F15S2 ELISA
Kit, Bovine D3S48E ELISA Kit, Bovine DNF15S2 ELISA Kit, Bovine N-acylaminoacyl-peptide hydrolase ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine acylamino-acid-releasing enzyme,AARE concentrations
in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to AARE. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AARE and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain AARE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AARE in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

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