Bovine Melanocyte protein PMEL, PMEL ELISA KIT

Bovine Melanocyte protein PMEL, PMEL ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Bovine PMEL ELISA KIT ,Bovine melanocyte protein Pmel 17 ELISA KIT ,Bovine D12S53E ELISA KIT ,Bovine ME20 ELISA KIT ,Bovine ME20-M ELISA KIT ,Bovine ME20M ELISA KIT ,Bovine P100 ELISA KIT ,Bovine PMEL17 ELISA KIT ,Bovine SIL ELISA KIT ,Bovine SILV ELISA KIT ,Bovine gp100 ELISA KIT ,Bovine melanocyte protein mel 17 ELISA KIT ,Bovine melanocytes lineage-specific antigen GP100 ELISA KIT ,Bovine melanoma-associated ME20 antigen ELISA KIT ,Bovine melanosomal matrix protein17 ELISA KIT ,Bovine silver locus protein homolog ELISA KIT ,Bovine silver ELISA KIT ,Bovine  mouse ELISA KIT ,Bovine  homolog of ELISA KIT ,Bovine premelanosome protein ELISA KIT

Intended use

Bovine PMEL ELISA kit allows for the in vitro quantitative determination of Bovine PMEL concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Bovine PMEL ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PMEL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PMEL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PMEL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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