Bovine Myc proto-oncogene protein, MYC ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Bovine MYC ELISA KIT ,Bovine c-Myc ELISA KIT , Bovine v-myc avian myelocytomatosis viral oncogene homolog ELISA KIT ,Bovine v-myc myelocytomatosis viral oncogene homolog ELISA KIT ,Bovine proto-oncogene c-Myc ELISA KIT ,Bovine myc-related translation/localization regulatory factor ELISA KIT ,Bovine MRTL ELISA KIT ,Bovine MYCC ELISA KIT ,Bovine bHLHe39 ELISA KIT ,Bovine avian myelocytomatosis viral oncogene homolog ELISA KIT ,Bovine class E basic helix-loop-helix protein 39 ELISA KIT ,Bovine transcription factor p64 ELISA KIT
Intended use
Bovine MYC ELISA kit allows for the in vitro quantitative determination of Bovine MYC concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Bovine MYC ELISA kit has been pre-coated with an antibody specific to MYC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MYC and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MYC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MYC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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